MARCKS (phospho Ser162) Antibody

Product Specifications

Background

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

NCBI Gene ID

4082

Swiss Prot

P29966

Accession Number

NP_002347.5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MARCKS (Phospho-Ser162) antibody was raised against a peptide sequence around phosphorylation site of serine 162 (K-K-S (p) -F-K) derived from Human MARCKS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

80 kDa

Specificity

This antibody detects endogenous level of MARCKS only when phosphorylated at serine 162.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

MARCKS

NCBI Official Name

Myristoylated alanine-rich protein kinase C substrate

NCBI Organism

Homo sapiens

Background Reference 01

Pariser H, et al. Proc Natl Acad Sci U S A 2005 Aug 30; 102 (35) : 12407-12412Nagumo H, et al. Biochem Biophys Res Commun 2001 Jan 26; 280 (3) : 605-609Yamamoto H, et al. Arch Biochem Biophys 1998 Nov 15; 359 (2) : 151-159