CD273 Antibody [TY25]

Product Specifications

Background

The TY25 antibody reacts with mouse B7-DC (PD-L2), a 42 kDa glycoprotein of the B7 family of the immunoglobulin superfamily. B7-DC is expressed by macrophages, monocytes, and sub-populations of dendritic cells. B7-DC has similarities with the rest of the B7 family, but it does not bind to CD28/CD152. The receptor for B7-DC is PD-1, which contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), and is expressed on activated B and T cells. The interaction between B7-DC and its receptor seems to downregulate immune responses. However, B7-DC seems to be a costimulator of T lymphocytes, reacting with a receptor different than PD-1. B7-DC can bind and stimulate dendritic cells. It is also the first B-7 ligand involved in bidirectional communication. The TY25 monoclonal antibody blocks the binding of B7-DC to its receptor (PD-1) and can be used for studying the interaction between the antigen presenting cells and T lymphocytes.

NCBI Gene ID

58205

Swiss Prot

Q9WUL5

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

TY25

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity, Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

The TY25 antibody reacts with mouse B7-DC (PD-L2), a 42 kDa glycoprotein of the B7 family of the immunoglobulin superfamily.

Symbol

Pdcd1lg2

NCBI Official Name

Programmed cell death 1 ligand 2

NCBI Organism

Mus musculus

Background Reference 01

Matsumoto, M., Kikkawa, S., Kohase, M., Miyake, K., Seya, T. (2002) . Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling.Biochemical and biophysical research communications,293 (5), 1364-1369.

Background Reference 02

Schreiner, B., Voss, J., Wischhusen, J., Dombrowski, Y., Steinle, A., Lochm, H., ... Wiendl, H. (2006) . Expression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.The FASEB journal,20 (1), 118-120.

Background Reference 03

Oshiumi, H., Matsumoto, M., Funami, K., Akazawa, T., Seya, T. (2003) . TICAM-1, an adaptor molecule that participates in Toll-like receptor 3mediated interferon--beta- induction.Nature immunology,4 (2), 161-167.