IkB a (Ab 42) Antibody

Product Specifications

Background

Inhibits the activity of dimeric NF-κ -B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

NCBI Gene ID

4792

Swiss Prot

P25963

Accession Number

NP_065390.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IkB-a (Ab-42) antibody was raised against a peptide sequence around aa. 40~44 (E-E-Y-E-Q) derived from Human IkappaB-α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

39 kDa

Specificity

This antibody detects endogenous level of total IkappaB-α protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

NFKBIA

NCBI Official Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

NCBI Organism

Homo sapiens

Background Reference 01

Beraud C, et al. (1999) Proc Natl Acad Sci U S A 96 (2) : 429-434. Sundstrom S, et al. (2005) J Virol 79 (4) : 2230-2239. Liu L, et al. (1998) Mol Cell Biol 18 (7) : 4221-4234. Shrivastava A, et al. (1998) J Virol 72 (12) : 9722-9728. Franzoso G, et al. (1997) Genes Dev 11 (24) : 3482-3496.