STAT6 (Ab 641) Antibody

Product Specifications

Background

STAT6 is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor-associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. By screening an embryonic lung fibroblast cDNA library with a wildtype STAT6 probe, 2 variant cDNAs were identified, which were termed STAT6B and STAT6C, encoding an N-terminal 110-amino acid truncation and a 27-amino acid deletion in the SH2 domain, respectively. All the three variants are ubiquitously expressed with STAT6B expression greatest in spleen and STAT6C expression greatest in lung. STAT6B resembles an attenuated STAT6, but that STAT6C inhibits IL-4-mediated mitogenesis and cell surface antigen expression, and is not tyrosine phosphorylated

NCBI Gene ID

6778

Swiss Prot

P42226

Accession Number

NP_003144.3

Host

Rabbit

Reactivity

Human, Rat

Immunogen

STAT6 (Ab-641) antibody was raised against a peptide sequence around aa.639~643 (R-G-Y-V-P) derived from Human STAT6.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

110 kDa

Specificity

This antibody detects endogenous level of total STAT6 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

STAT6

NCBI Official Name

Signal transducer and activator of transcription 6, interleukin-4 induced

NCBI Organism

Homo sapiens

Background Reference 01

Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343. Takeda K, et al. (1996) Nature. 380: 627-630.