TPX2 Antibody

Product Specifications

Background

TPX2 is a spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

NCBI Gene ID

22974

Swiss Prot

Q9ULW0

Accession Number

Q9ULW0

Host

Rabbit

Reactivity

Human

Immunogen

This TPX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the Central region of human TPX2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

86 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

TPX2

NCBI Official Name

Targeting protein for Xklp2

NCBI Organism

Homo sapiens

Background Reference 01

Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :

Background Reference 02

Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

Background Reference 03

Bibby, R.A., et al. J. Biol. Chem. 284 (48) :33177-33184 (2009)

Background Reference 04

Shigeishi, H., et al. Int. J. Oncol. 34 (6) :1565-1571 (2009)

Other Product Names

Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519