JARID1B Antibody

Product Specifications

Background

Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.

NCBI Gene ID

10765

Swiss Prot

Q9UGL1

Accession Number

Q9UGL1

Host

Rabbit

Reactivity

Mouse

Immunogen

This JARID1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-224 amino acids from the N-terminal region of human JARID1B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Concentration

Batch dependent

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

176 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

KDM5B

NCBI Official Name

Lysine-specific demethylase 5B

NCBI Organism

Homo sapiens

Background Reference 01

Kim, J., et al. Biochem. Biophys. Res. Commun. 401 (3) :412-416 (2010)

Background Reference 02

Krishnakumar, R., et al. Mol. Cell 39 (5) :736-749 (2010)

Background Reference 03

Yao, W., et al. Biochem. Biophys. Res. Commun. 396 (2) :323-328 (2010)

Background Reference 04

Roesch, A., et al. Cell 141 (4) :583-594 (2010)

Other Product Names

Lysine-specific demethylase 5B, 11411-, Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1, RBP2-H1, KDM5B, JARID1B, PLU1, RBBP2H1