PARP-10 Antibody [5H11]

Product Specifications

Background

PARP-10. PARP-10 mono (ADP-ribosyl) ates (MARylates) NEMO (NF-kappaB essential modulator), which results in reduced NEMO polyubiquitylation and thus decreased NF-kappaB signaling. Overexpression of PARP-10 was shown to lead to apoptosis. PARP-10 knockdown leads to increased cell survival. Additional substrates of PARP-10, such as GSK-3beta were identified and PARP-10-mediated mono (ADP-ribosyl) ation (MARylation) was shown to be involved in regulating multiple processes, including Wnt signaling.

NCBI Gene ID

84875

Swiss Prot

Q53GL7

Accession Number

116248563

Host

Rat

Reactivity

Human

Immunogen

Recombinant human PARP-10 [ARTD10] (aa 1-907) fused to a GST-tag.

Clonality

Monoclonal

Clone

5H11

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Innate Immunity, Obesity

Concentration

Batch dependent

Buffer

Liquid. Cell culture supernatant containing RPMI, 10% FCS and 0.05% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Stable for at least 1 year after receipt when stored at +4˚ C.

Fragment

IgG1

Specificity

Recognizes human PARP-10 [ARTD10] with an epitope between aa 256-407, containing the glycine rich (G-rich) region.

Applications Notes

Immunocytochemistry: (1:50-1:200) . Immunoprecipitation: (1:10) . Western Blot: (1:50) . Optimal conditions must be determined individually for each application.