Mucin-5AC Antibody [SPM297]
Product Specifications
Background
This mAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC) . Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
NCBI Gene ID
4586
Swiss Prot
P98088
Host
Mouse
Reactivity
Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken
Immunogen
An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient was used as the immunogen for this Mucin-5AC antibody.
Clonality
Monoclonal
Clone
SPM297
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=34-137
Fragment
IgG1, kappa
Specificity
Does not react with Cow
Applications Notes
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Symbol
MUC5AC
NCBI Official Name
Mucin 5AC, oligomeric mucus/gel-forming
NCBI Organism
Homo sapiens
Other Product Names
MUC5AC, Gastric mucin, Major airway glycoprotein, Muc5/5ac, Mucin-5AC, LeB, Lewis B blood group antigen, Tracheobronchial mucin, TBM, MUC-5AC, MUC5
Tested Applications
Flow, IF, IHC
Physical Properties
Liquid
Curated Selection
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