GLIS3 Antibody

Product Specifications

Background

GLIS3 is a member of the GLI-similar zinc finger protein family and has five C2H2-type zinc finger domains. GLIS3 functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH) .

NCBI Gene ID

169792

Swiss Prot

Q1PHJ8

Accession Number

NP_689842

Host

Rabbit

Reactivity

Human, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GLIS3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GLIS3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

84 kDa, 100 kDa

Applications Notes

GLIS3 antibody can be used for detection of GLIS3 by ELISA at 1:62500. GLIS3 antibody can be used for detection of GLIS3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.