ATF4 Antibody

ATF4s a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2) . The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATF4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

ATF4 antibody can be used for detection of ATF4 by ELISA at 1:62500. ATF4 antibody can be used for detection of ATF4 by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.