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NT5C3 Antibody

Product Specifications

Background

Pyrimidine 5-prime-nucleotidase (P5N), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2) . The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology. Pyrimidine 5-prime-nucleotidase (P5N; EC 3.1.3.5), also called uridine 5-prime monophosphate hydrolase (UMPH), catalyzes the dephosphorylation of the pyrimidine 5-prime monophosphates UMP and CMP to the corresponding nucleosides. There are 2 isozymes of pyrimidine 5-prime nucleotidase in red blood cells, referred to as type I (UMPH1) and type II (UMPH2; MIM 191720) . The 2 enzymes are not separable by electrophoresis in humans but have distinct kinetic properties, and the proteins show no homology.

NCBI Gene ID

51251

Swiss Prot

Q9H0P0-1

Accession Number

NP_001002009

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NT5C3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NT5C3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

34 kDa

Applications Notes

NT5C3 antibody can be used for detection of NT5C3 by ELISA at 1:1562500. NT5C3 antibody can be used for detection of NT5C3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

NT5C3A

NCBI Official Name

5'-nucleotidase, cytosolic IIIA

NCBI Organism

Homo sapiens

Background Reference 01

Chondrogianni, N. (2007) Exp. Gerontol. 42 (9), 899-903.

Other Product Names

NT5C3, MGC27337, MGC87109, MGC87828, P5'N-1, PN-I, PSN1, UMPH, UMPH1, cN-III, p36, POMP, NT5C3, P5N-1, hUMP1

Tested Applications

ELISA, WB

Protein ID

70608080

Physical Properties

Liquid

Frequently Asked Questions

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