SPIB Antibody

Product Specifications

Background

SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1) . SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3) . It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN) -producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4) .

NCBI Gene ID

6689

Swiss Prot

Q01892

Accession Number

NP_003112

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell, Immunology

Purification

SPIB antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1312 - Human Prostate Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (88%)

Buffer

SPIB antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SPIB antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

Symbol

SPIB

NCBI Official Name

Spi-B transcription factor (Spi-1/PU.1 related)

NCBI Organism

Homo sapiens

Background Reference 01

Ray D, Bosselut R, Ghysdael J, et al. Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1. Mol. Cell Biol. 1992; 12:4297-304.

Background Reference 02

Nagy M, Chapuis B, and Matthes T. Expression of transcription factors Pu.1, Spi-B, Blimp-1, BSAP and oct-2 in normal human plasma cells and in multiple myeloma cells. Br. J. Haematol. 2002; 116:429-35.

Background Reference 03

Schotte R, Rissoan MC, Bendriss-Vermare N, et al. The transcription factor Spi-B is expressed in plasmacytoid DC precursors and inhibits T-, B-, and NK-cell development. Blood 2003; 101:1015-23.

Background Reference 04

Schotte R, Nagasawa M, Weijer K, et al. The ETS transcription factor Spi-B is required for human plasmacytoid dendritic cell development. J. Exp. Med. 2004; 200:1503-9.