Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

ATG5 Antibody

Product Specifications

Background

ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3) -like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

NCBI Gene ID

9474

Swiss Prot

A9UGY9

Accession Number

EAW48415

Host

Chicken

Reactivity

Human, Mouse

Immunogen

5031

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar ATG5 expression profile in human and mouse cell lines detected by two independent anti-ATG5 antibodies that recognize different epitopes, 5031 against N-terminus domain and 64-229 against human recombinant protein. ATG5 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5031

Field of Research

Autophagy

Purification

ATG5 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1406 - Mouse Spleen Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat (100%), Bovine: (94%), Pig: (94%)

Buffer

ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ATG5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=5031

Fragment

IgY

Specificity

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.

Isoform

Human ATG5 has 2 isoforms, including isoform L (275aa, 32.4kD) and isoform S (197aa, 22.9kD. This antibody detects only human isoform L. Mouse ATG5 has 1 isoform (275aa, 32.4kD) . Rat ATG5 has only one isoform identified so far (275aa, 32.4kD) .

Symbol

ATG5

Positive Control 2

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

ATG5 autophagy related 5 homolog (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Gozuacik and Kimchi. Oncogene 2004; 23:2891-906.

Background Reference 02

Kisen et al. Carcinogenesis 1993; 14:2501-5.

Background Reference 03

Kamada et al. J. Cell. Biol. 2000; 150:1507-13.

Background Reference 04

Hanada et al. J. Biol. Chem.2007; 282:37298-302.

Other Product Names

ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE

Tested Applications

ELISA, WB, IHC-P

Protein ID

119568800

Physical Properties

Liquid

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

PRAMEF12 Double Nickase Plasmid (m2)
sc-429560-NIC-2 20 µg

PRAMEF12 Double Nickase Plasmid (m2)

Ask
View Details
Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit
MBS4502044-01 48 Tests

Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit

Ask
View Details
Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit
MBS4502044-02 96 Tests

Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit

Ask
View Details
Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit
MBS4502044-03 5x 96 Tests

Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit

Ask
View Details
Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit
MBS4502044-04 10x 96 Tests

Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit

Ask
View Details
FLJ20244 antibody - middle region
MBS3202145-01 0.1 mL

FLJ20244 antibody - middle region

Ask
View Details