UNG1 Antibody

UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.

Independent Antibody Validation in Cell lines (Figure 2) shows similar UNG1 expression profile in human cell lines detected by two independent anti-UNG1 antibodies that recognize different epitopes, 3863 against C-terminus domain and 3865 against central domain. UNG1 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3863 3865

UNG1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Human UNG1 has 2 isoforms, including isoform 1 (304aa, 34kD) and isoform 2 (313aa, 35kD) . Mouse UNG1 has 2 isoforms, including isoform 1 (295aa, 33kD) and isoform 2 (306aa, 34kD) . Rat UNG1 has one isoform (303aa, 34kD) . 3865 can detect human, mouse and rat.