Ric8a (NM_053194) Mouse Recombinant Protein

Product Specifications

Background

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G (i) -alpha protein, possibly leading to release G (i) -alpha-GTP and NuMA proteins from the NuMA-GPSM2-G (i) -alpha-GDP complex. Also acts as an activator for G (q) -alpha (GNAQ) protein by enhancing the G (q) -coupled receptor-mediated ERK activation (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse RIC8 guanine nucleotide exchange factor A (Ric8a), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

AI114950; RIC-8; Ric8

Gene ID

101489

UniProt

Q3TIR3

Accession Number mRNA

NM_053194

Chromosomal Location

7 F4

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

59.8 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.