Wee2 (NM_201370) Mouse Recombinant Protein

Product Specifications

Background

Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse WEE1 homolog 2 (S. pombe) (Wee2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

Gm1065; Wee1B

Gene ID

381759

UniProt

Q66JT0

Accession Number mRNA

NM_201370

Chromosomal Location

6 B1

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

62.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.