Chaf1b (NM_028083) Mouse Recombinant Protein

Product Specifications

Background

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse chromatin assembly factor 1, subunit B (p60) (Chaf1b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2600017H24Rik; C76145; CAF-I p60; CAF-Ip60; CAF1; CAF1A; CAF1P60; MPHOSPH7

Gene ID

110749

UniProt

Q9D0N7

Accession Number mRNA

NM_028083

Chromosomal Location

16 54.96 cM

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

63.6 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.