Rec114 (NM_028598) Mouse Recombinant Protein
Product Specifications
Background
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination (PubMed:20551173, PubMed:27723721) . Probably acts by forming a complex with IHO1/CCDC36 and MEI4, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis (PubMed:27723721) .[UniProtKB/Swiss-Prot Function]
Overview
Purified recombinant protein of Mouse REC114 meiotic recombination protein (Rec114), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
Synonyms
2410076I21Rik; 4930527A11Rik
Gene ID
73673
UniProt
Q9CWH4
Accession Number mRNA
NM_028598
Chromosomal Location
9
Expression System
HEK293T
Tag
C-MYC/DDK
Concentration
>50 ug/mL as determined by microplate BCA method
Purity
> 80% as determined by SDS-PAGE and Coomassie blue staining
Form
Liquid
Buffer
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Molecular Weight
28.2 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Rec114
Species
Mouse
Protein ID
NP_082874
Gene ID URL
https://www.ncbi.nlm.nih.gov/gene/73673
Uniprot URL
https://www.uniprot.org/uniprot/Q9CWH4
Accession Number mRNA URL
https://www.ncbi.nlm.nih.gov/nuccore/NM_028598
Protein ID Link
https://www.ncbi.nlm.nih.gov/nuccore/NP_082874
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