Rec114 (NM_028598) Mouse Recombinant Protein

Product Specifications

Background

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination (PubMed:20551173, PubMed:27723721) . Probably acts by forming a complex with IHO1/CCDC36 and MEI4, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis (PubMed:27723721) .[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse REC114 meiotic recombination protein (Rec114), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

2410076I21Rik; 4930527A11Rik

Gene ID

73673

UniProt

Q9CWH4

Accession Number mRNA

NM_028598

Chromosomal Location

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

28.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.