VISTA Protein, Cynomolgus, Recombinant (hFc Tag)

Product Specifications

Background

VSIR (V-Set Immunoregulatory Receptor, also known as VISTA) is a Protein Coding gene. VISTA is an immunoregulatory receptor that inhibits the T-cell response. It may promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. VSIR, or V-set immunoregulatory receptor, could be involved in the pathogenesis of chronic rhinosinusitis with nasal polyps. V-domain Immunoglobulin Suppressor of T cell Activation (VISTA) is an inhibitory immune-checkpoint molecule that suppresses CD4+ and CD8+ T cell activation when expressed on antigen-presenting cells. VSIR is broadly expressed in the spleen, bone marrow, and other tissues. Diseases associated with VSIR include Ichthyosis, Congenital, Autosomal Recessive 6, and Monckeberg Arteriosclerosis. An important paralog of this gene is VSIG8.

Overview

A DNA sequence encoding the cynomolgus B7H5 (XP_005565644.1) (Met1-Ala194) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

C9H10orf54 Protein, Cynomolgus

Gene Name

V-set immunoregulatory receptor

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Immobilized human IGSF11-His (PH50174M2) at 2 μg/mL (100 μL/well) can bind Cynomolgus B7H5-hFc (PK54033M4), the ECof Cynomolgus B7H5-hFc (PK54033M4) is 0.4-1.3 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

B7 Family proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

Molecular Weight

The recombinant cynomolgus B7H5 consists of 400 amino acids and predicts a molecular mass of 44.9 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.