CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Pro 217) was fused with a polyhistidine tag at the C-terminus.

Gene Name

Fc fragment of IgG, low affinity IIb, receptor (CD32)

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.Immobilized cynoCD32B-His (Cat:PK54018M2) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The ECof biotinylated human IgG1 is 0.17-0.41 μg/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Fc receptor proteins, Immunotherapy proteins, CD Molecule proteins, Receptor Proteins

Molecular Weight

The recombinant cynomolgus CD32b consists of 184 amino acids and has a calculated molecular mass of 20.9 kDa. The apparent molecular mass of the recombinant protein is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.