CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His Tag)
Product Specifications
Gene Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Expression System
HEK293 Cells
Tag
C-His
Field of Research
Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA.Immobilized cynoCD32B-His (Cat:PK54018M2) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The ECof biotinylated human IgG1 is 0.17-0.41 μg/ml.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Fc receptor proteins, Immunotherapy proteins, CD Molecule proteins, Receptor Proteins
Molecular Weight
The recombinant cynomolgus CD32b consists of 184 amino acids and has a calculated molecular mass of 20.9 kDa. The apparent molecular mass of the recombinant protein is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PK54018M2.pdf
Symbol
FCGR2B
Species
Cynomolgus
Protein ID
AAL92097.1
Overview
A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Pro 217) was fused with a polyhistidine tag at the C-terminus.
AA Sequence
Met1-Pro217
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/AAL92097.1
Available Sizes
Curated Selection
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