Brk Protein, Mouse, Recombinant (His & GST Tag)

Tyrosine kinase (PTKs) is a protein that carries out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demonstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase (PTKs) -6/ BRK is a cytoplasmic non-receptor protein kinase that may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase (PTKs) -6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase (PTKs) -6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.

Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant mouse PTK6/GST chimera consists of 688 amino acids and has a calculated molecular mass of 79.8 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.