GLUD1/GLUD2 Antibody
GLUD1/GLUD2 Antibody
Product Specifications
Product Name Alternative
GLUD1, glutamate dehydrogenase 1, GDH, GDH1, GLUD, MGC132003, glutamate dehydrogenase (NAD (P) +), GLUD2, glutamate dehydrogenase 2, GDH2, GLUDP1, glutamate dehydrogenase pseudogene 1
UniProt
P00367
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-ESEEQKRNRVRGILR
Target
GLUD1 / GLUD2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration: 3-5μg/mL.Immunofluorescence: Customer found particulate cytoplasm staining in HeLa. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IF, IHC, WB
NCBI Accession Number
NP_005262.1, NP_036216.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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