CD200R Protein, Mouse, Recombinant (His Tag)

Product Specifications

Background

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cell surface glycoprotein CD200 receptor 1 (CD200R1) is an isoform of CD200 receptors that is expressed on cells of the myeloid lineage. CD200R1 is a receptor for the OX-2 membrane glycoprotein. The receptor-substrate interaction may serve as a myeloid downregulatory signal.

Overview

A DNA sequence encoding the extracellular domain (Met 1-Pro 238) of mouse CD200R1 (NP_067300.1) was expressed, with a C-terminal polyhistidine tag.

Synonyms

CD200R Protein, Mouse; Mox2r Protein, Mouse; OX2R Protein, Mouse

Gene Name

CD200 receptor 1

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Neuroinflammation Cancer Drug Targets

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its ability to bind mouse CD200 in a functional ELISA.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Immunotherapy proteins, Cancer proteins, Immune Checkpoint Proteins

Molecular Weight

The recombinant mouse CD200R1 consists of 224 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCD200R1 is approximately 55-65 kDa due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.