Sorcin/SRI Protein, Human, Recombinant (His & GST Tag)
Product Specifications
Background
Sorcin was originally identified in multidrug-resistant cells. It is a calcium-binding protein. Sorcin modulates excitation-contraction coupling in the heart, contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Sorcin is overexpressed in the multi-drug resistant chinese hamster ovary cell line CHRC5 and a variety of multidrug-resistant tumor cell lines, but overexpression is not a sufficient or necessary condition for the acquisition of the multidrug-resistant phenotype.
Synonyms
CP-22 Protein, Human; CP22 Protein, Human; SCN Protein, Human; V19 Protein, Human
Gene Name
Sorcin
UniProt
P30626
Expression System
Baculovirus-Insect Cells
Tag
N-GST & His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human SRI /GST chimera consists of 435 amino acids and has a calculated molecular mass of 49.5 kDa. The recombinant protein migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50921I6.pdf
Symbol
SRI
Species
Human
Overview
A DNA sequence encoding the human SRI (P30626) (Met1-Val198) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/P30626
AA Sequence
Met1-Val198
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