Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag)

Product Specifications

Background

Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest) . It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in the reduction of cell movement and proliferation.

Overview

A DNA sequence encoding the human NECTIN3 (NP_056295.1) (Met1-Asp400) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

CD113 Protein, Human; CDw113 Protein, Human; DKFZp566B0846 Protein, Human; FLJ90624 Protein, Human; nectin-3 Protein, Human; PPR3 Protein, Human; PRR3 Protein, Human; PVRL3 Protein, Human; PVRR3 Protein, Human

Gene Name

Poliovirus receptor-related 3

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag) (Cat:PH50410M2) at 2 μg/ml (100 μl/well) can bind Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:PH50814M4), the ECof Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag) is 30-140 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Biological Target proteins, Immune Checkpoint Proteins

Molecular Weight

The recombinant human NECTIN3 consists of 581 amino acids and predicts a molecular mass of 64.4 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.