Recombinant Pectobacterium cypripedii Gluconate 2-dehydrogenase flavoprotein, partial
Product Specifications
Product Name Alternative
GA 2-DH dehydrogenase subunit
Abbreviation
Recombinant Pectobacterium cypripedii Gluconate 2-dehydrogenase flavoprotein, partial
UniProt
O34214
Expression Region
451-615aa
Organism
Pantoea cypripedii (Pectobacterium cypripedii) (Erwinia cypripedii)
Target Sequence
ADTYNHHISMDAHGAHQSYRANYLDLDPNYKNVYGQPLLRMTFDWQDNDIRMAQFMVGKMRKITEAMNPKMIIGGAKGPGTHFDTTVYQTTHMSGGAIMGEDPKTSAVNRYLQSWDVPNVFVPGASAFPQGLGYNPTGMVAALTYWSAKAIREQYLKNPGPLVQA
Tag
C-terminal 6xHis-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Part of the heterotrimer that catalyzes the conversion of D-gluconate to 2-dehydro-D-gluconate. This subunit functions as the dehydrogenase
Endotoxin
Not test
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
25.3 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Frequently Asked Questions
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