Thrombin Inhibitor Screening Assay Kit

Thrombin (also known as factor IIa, activated blood-coagulation factor II, EC 3.4.21.5 or fibrogenase) is a serine protease crucial for blood coagulation. Thrombin is synthetized in the inactive form prothrombin, and it is cleaved by activated Factor X (Xa) . It activates factor XI, VIII, V XIII, and converts fibrinogen to fibrin by cleaving fibrinogen chains into monomers. Activated factor XIII (XIIIa) increases the stability of the clot by forming covalent bonds between fibrin molecules. In addition, thrombin leads to platelet activation and aggregation. Interestingly, thrombin is also involved in a negative feedback mechanism, by acting as an inhibitor of the coagulation cascade when bound to thrombomodulin. Thrombin is involved in the formation of blood clots in arteries, which can result in cerebral ischemia and stroke. It is also involved in atherosclerosis via angiogenesis (vascular cell recruitment to the plaque), and inflammation. The use of small molecule inhibitors for thrombin is a viable therapy in cardiovascular complications

96 reactionsCatalog #NameAmountStorage82887Human alpha thrombin*5 µg-80°C82888PR Substrate 250 µl-80°C82773PR-02 Buffer10 ml-80°C79963Transparent 96-well microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.384 reactionsCatalog #NameAmountStorage82887Human alpha thrombin*2 x 5 µg-80°C82888PR Substrate 22 x 50 µl-80°C82773PR-02 Buffer2 x 10 ml-80°C79962Transparent 384-well microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

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