COX1 Inhibitor Screening Assay Kit

COX1 (cyclooxygenase 1), also known as prostaglandin-endoperoxide synthase 1 (PTGS), is a peroxidase involved in the formation of prostaglandins by converting arachidonic acid to prostaglandin H2.COX1 is expressed constitutively in most cells and participates in tissue homeostasis.  COX-1 promotes cytoprotection of the gastric mucosa by increasing the formation of mucus and bicarbonate, reducing acid and pepsin secretion and keeping appropriate blood flow. It is involved in the formation of thromboxane A2 (TxA2) in platelets, which stimulates aggregation and vasoconstriction. COX1 and COX2 differ in position 523, with COX2 having a valine and COX1 an isoleucine residue, making the development of specific inhibitors a challenge. The use of COX inhibitors or NSAIDs (non-steroidal anti-inflammatory drugs) is common to manage pain and inflammation. Classical NSAIDs, such as aspirin and ibuprofen, can result in damage to the gastrointestinal system. The use of low dose aspirin can help prevent atherothrombosis and the discovery of other compounds able to inhibit COX1 may prove beneficial as coadjuvants in cardiovascular disease therapy.

Catalog #NameAmountStorage71110COX1, FLAG-His-Tags*70 µg-80°CAmplex™ Red100 µl-80°CArachidonic Diluent250 µl-80°CCOX Assay Buffer10 ml-80°C100% Ethanol1 ml-80°C7968596-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

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