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Anti-S100A5 Antibody

Our Anti-S100A5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N176/35. It is KO validated, detects mouse and rat S100A5, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Product Specifications

Background

Protein S100A5 is encoded by the gene S100A5 and a member of the S100 family of proteins. S100A5 is a calcium-binding cytosolic proteins and is also a member of the damage-associated molecular pattern molecules (DAMPs) family, a group of proteins that are released from the extracellular or intracellular space following tissue injury or cell death. S100A5 is localized in the cytoplasm and implicated in the regulation of a number of cellular processes such as cell cycle progression and extracellular signal transduction. S100A5 has been found in brain and kidney. Diseases associated with this gene include Astrocytoma.

Short Description

Our Anti-S100A5 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N176/35. It is KO validated, detects mouse and rat S100A5, and is purified by protein a chromatography. It is great for use in WB, ICC, ELISA.

Synonyms

Protein S100-A5 (Protein S-100D) (S100 calcium-binding protein A5)

Gene Name

S100a5 S100d

UniProt

P63083

Host

Mouse

Reactivity

Mouse and Rat

Immunogen

Fusion protein amino acids 1-93 (full-length) of rat S100A (accession number P63083) produced recombinantly in E. Coli

Target

S100A5

Clonality

Monoclonal

Isotype

IgG1

Clone

N176/35

Type

Primary Antibody

Applications

WB, ICC, ELISA

Field of Research

Cell Signaling

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Molecular Weight

10 kDa

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Specificity

Does not cross-react with S100A4 or S100A6 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Recommended Usage

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Physical Properties

Liquid

Immunogen Species

Rat

ICC Quality Control

1

Conjugate

Unconjugated

RRID

AB_2315917

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