What is the catalog number for Anti-PSD-95 Antibody?

The catalog number for Anti-PSD-95 Antibody is 75-028-020.

What sizes are available for Anti-PSD-95 Antibody?

Anti-PSD-95 Antibody is available in the following size(s): 20 µL.

Is Anti-PSD-95 Antibody in stock?

Anti-PSD-95 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-PSD-95 Antibody

Our Anti-PSD 95 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Product Specifications

Background

PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003) . Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Short Description

Our Anti-PSD 95 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K28/43. It is KO validated, detects chicken, human, mouse, non-human primate, and rat PSD 95, and is purified by protein a chromatography. It is great for use in WB, IHC, ICC, IP, Flow, ELISA.

Synonyms

Disks large homolog 4 (Postsynaptic density protein 95) (PSD-95) (Synapse-associated protein 90) (SAP-90) (SAP90)

Gene Name

DLG4 PSD95

UniProt

P78352

Host

Mouse

Reactivity

Chicken, Human, Mouse, Non-Human Primate, and Rat

Immunogen

Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (accession number P78352) produced recombinantly in E. Coli

Target

PSD 95

Clonality

Monoclonal

Isotype

IgG2a

Clone

K28/43

Type

Primary Antibody

Applications

WB, IHC, ICC, IP, Flow, ELISA

Field of Research

Scaffold Proteins

Concentration

1 mg/mL

Format

Purified by Protein A chromatography

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Molecular Weight

95-110 kDa (varies with cell background due to phosphorylation)

Shipping Conditions

Shipped on ice packs

Storage Conditions

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

IHC Dilution

1:500

WB Dilution

1:1000

IP Dilution

5 μg

Specificity

No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102

ICC Dilution

1:500

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.