7-AAD Red Fluorescent Live/Dead Stain

1. Add 1 part Antigen-Down HRP Conjugate Stabilizer to 4 parts deionized water and mix for 5-15 minutes. 2. Add the anti-IgG-HRP conjugate directly into the conjugate diluent to a working 1X dilution and mix for 5-15 minutes. 3. Pipette diluted conjugate onto the plate, or store protected from light at 2-8°C for use at a later date. 1X working conjugate dilutions can be stored at 2-8°C and protected from light, providing a useful shelf-life of 6-12 months.