Anti-MPXV E8L Human IgG ELISA Kit
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV E8L antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-MPXV E8L Human IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-MPXV E8L Human IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Specifications
Applications
Used for the quantitative determination of Anti-MPXV E8L Human IgG concentration in serum and plasma.
Stability
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%.
Shipping Conditions
2-8 °C
Notes
For Research Use Only.
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