HighYield T7 P&L RNA Synthesis Kit

HighYield T7 P&L RNA Synthesis Kit is designed to produce large amounts of RNA via in vitro transcription with a modified T7 RNA Polymerase. Proline 266 has been replaced by leucine (P266L) which has been associated with: decreased abortive transcription. increased 5' homogeneity of transcripts synthesized from A-initiating phi2.5 promoter; increased 5' incorporation efficiency of GTP analogs The resulting RNA can subsequently be used for a variety of appli-cations such as microinjection, transfection or in vitro translation experiments, structure/function studies or probes for in situ hy-bridization and Northern Blot experiments. One kit contains sufficient reagents for 50 reactions of 20 μl each (7.5 mM each NTP) . A 20 μl reaction yields about 140-160 μg RNA after 30 min incubation (1 μg T7 control template, 1.4 kb RNA transcript) . Yields may however vary depending on the template (promoter design, sequence length, secondary structure formation) .