T7 P&L RNA Polymerase HC

Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA poly-merase (99 kDa) that catalyzes in vitro RNA synthesis from a DNA sequence containing a T7 phage promoter. The highly concentrated version and modified T7 RNA Poly-merase HC is ideally suited for set-up and optimization of high yield in vitro transcription reactions. Proline 266 has been replaced by leucine (P266L) which has been associated with: decreased abortive transcription. increased 5' homogeneity of transcripts synthesized from A-initiating phi2.5 promoter; increased 5' incorporation efficiency of GTP analogs The provided T7 IVT Set-up Buffer, MgCl 2and DTT solutions enable testing of different reaction conditions such as nucleotide/MgCl 2 concentrations. Recommendation for assay set-up with minimal optimization: HighYield T7 P&L RNA Synthesis Kit (#orb653830) .