Photosensitizer-8

Photosensitizer-8 (Compound 4) is a derivative of 2-anthrol and serves as a photosensitizer activated by alkaline phosphatase (ALP) . In the presence of ALP, it undergoes a phosphate ester hydrolysis reaction, rapidly converting into the active photosensitizer 2-anthrol. Upon irradiation, Photosensitizer-8 exhibits cytotoxicity against cancer cells with ALP overexpression, such as HeLa, A549, and HCT116, with IC50 values of 14.3 μM, 21.6 μM, and 17.5 μM respectively, while showing no significant cytotoxicity towards normal lung fibroblasts (WI-38) with IC50 ≥ 30 μM. Photosensitizer-8 is applicable in researching photodynamic therapy for cancers associated with ALP overexpression.