SARS-CoV-2 3CLpro-IN-34

SARS-CoV-2 3CLpro-IN-34 (Compound 55) is a highly effective non-covalent inhibitor of the SARS-CoV-2 3CLpro protease, with an IC50 of 1.9 μM. It also inhibits the SARS-CoV-1 3CL protease, displaying an IC50 of 3.2 μM, and shows strong selectivity for host cysteine proteases such as cathepsins L/K and calpain. Furthermore, SARS-CoV-2 3CLpro-IN-34 exhibits antiviral activity in SARS-CoV-2 infected cells, with an EC50 of 25 μM, is unaffected by P-gp inhibitors, and does not cause significant cytotoxicity. This compound can be utilized in the study of SARS-CoV-2 infections.