Mouse Human IgM (class specific), conjugated with FITC Antibody
Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immuno- fluorescence staining of cytoplasmic IgM and cell membrane IgM. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50. These data should be interpreted as general recommendations only.
Product Specifications
Reactivity
Human
Clonality
Monoclonal
Clone
NI 179
Conjugation
FITC
Source
Highly purified IgM isolated from pooled human serum
Field of Research
Stem Cells
Form
Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH7.2) . No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Storage Conditions
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2) . Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
Notes
For research use only.
Host or Source
Mouse
Isotype
IgG1
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