DBCO-PEG4-FLAG
DBCO-PEG4-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any Azide-functionalized molecule via Cu (I) -free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) . The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. DBCO-FLAG (without PEG-linker) has been successfully used for the detection of cell-surface Azides (final concentration: 100 M) or labeling of Azide-functionalized micelles (amount: 100 nmol) . These concentrations may serve as a starting point for individual assay set-up.
Product Specifications
Product Name Alternative
Dibenzylcyclooctyne-PEG4-FLAGtag (DYKDDDDK)
Purity
95% (HPLC)
Form
Solid; Color: white to off-white
Solubility
Water, PBS (up to 30 mM tested)
Molecular Formula
C80H107N15O30S
Molecular Weight
Theoretical MW: 1790.85 g/mol; Detected MW: 1789.70 g/mol
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: max 308 nm, 13.8.0 L mmol-1cm-1.
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