What is the catalog number for Aminoallyl-dUTP - Solid?

The catalog number for Aminoallyl-dUTP - Solid is orb1733888.

What sizes are available for Aminoallyl-dUTP - Solid?

Aminoallyl-dUTP - Solid is available in the following size(s): 5 mg.

Is Aminoallyl-dUTP - Solid in stock?

Aminoallyl-dUTP - Solid is currently made to order / not held in immediate stock. Contact us for lead time.

Aminoallyl-dUTP - Solid

Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.

Product Specifications

CAS Number

116840-18-7

Product Name Alternative

5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, Sodium salt

Purity

≥ 95% (HPLC)

Form

Solid; Color: white to off-white

Smiles

NC/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O

Molecular Formula

C12H20N3O14P3

Molecular Weight

Theoretical MW: 523.22 g/mol (free acid) ; Detected MW: 523.02 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: PCR with Taqpolymerase in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .