Biotin-16-dUTP

Biotin-16-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine. Recommended Biotin-16-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-16-dUTP/50% dTTP.

Product Specifications

Product Name Alternative

Biotin-16- (5-aminoallyl) -dUTP, Biotinyl-ε-aminocaproyl-γ-aminobutyryl-5- (3-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 98% (HPLC)

Form

Filtered solution (30 kDa) in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@H]3C[C@@H] ([C@H] (O3) COP (=O) (OP (=O) (OP (=O) (O) O) O) O) O) c1) NC (=O) N2

Molecular Formula

C32H52N7O18P3S

Molecular Weight

Theoretical MW: 947.78 g/mol (free acid) ; Detected MW: 947.23 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Incorporation into RNA by 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) . Spectroscopic Propertie: λmax 240 nm, ε 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .