Cep19 (NM_025892) Mouse Recombinant Protein

Product Specifications

Background

Required for ciliation. Recruits the RABL2B GTPase to the ciliary base to initiate ciliation. After specifically capturing the activated GTP-bound RABL2B, the CEP19-RABL2B complex binds intraflagellar transport (IFT) complex B from the large pool pre-docked at the base of the cilium and thus triggers its entry into the cilia. Involved in the early steps in cilia formation by recruiting the ciliary vesicles (CVs) to the distal end of the mother centriole where they fuse to initiate cilium assembly. Involved in microtubule (MT) anchoring at centrosomes.[UniProtKB/Swiss-Prot Function]

Overview

Purified recombinant protein of Mouse centrosomal protein 19 (Cep19), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

Synonyms

1500031L02Rik; AI428934; AL022620

Gene ID

66994

UniProt

Q9CQA8

Accession Number mRNA

NM_025892

Chromosomal Location

16 B2

Expression System

HEK293T

Tag

C-MYC/DDK

Concentration

>50 ug/mL as determined by microplate BCA method

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining

Form

Liquid

Buffer

25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Molecular Weight

19.2 kDa

Storage Conditions

Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.