SingleFlowEx CD138 Pacific Blue™

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

MI15

Applications

FC (QC tested)

Concentration

0.025 mg/mL

Format

Pacific Blue™

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Nadalin MR, Fregnani ER, Silva-Sousa YT, Perez DE: Syndecan-1 (CD138) and Ki-67 expression in odontogenic cystic lesions. Braz Dent J. 2011;22 (3) :223-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/21915520, *Jourdan M, Caraux A, Caron G, Robert N, Fiol G, Rème T, Bolloré K, Vendrell JP, Le Gallou S, Mourcin F, De Vos J, Kassambara A, Duperray C, Hose D, Fest T, Tarte K, Klein B: Characterization of a transitional preplasmablast population in the process of human B cell to plasma cell differentiation. J Immunol. 2011 Oct 15;187 (8) :3931-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/21918187, *Noll JE, Vandyke K, Hewett DR, Mrozik KM, Bala RJ, Williams SA, Kok CH, Zannettino AC: PTTG1 expression is associated with hyperproliferative disease and poor prognosis in multiple myeloma. J Hematol Oncol. 2015 Oct 6;8:106., URL: https://pubmed.ncbi.nlm.nih.gov/26445238/, *Atanackovic D, Hildebrandt Y, Templin J, Cao Y, Keller C, Panse J, Meyer S, Reinhard H, Bartels K, Lajmi N, Sezer O, Zander AR, Marx AH, Uhlig R, Zustin J, Bokemeyer C, Kröger N: Role of interleukin 16 in multiple myeloma. J Natl Cancer Inst. 2012 Jul 3;104 (13) :1005-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/22745469, *Krishnan SR, Luk F, Brown RD, Suen H, Kwan Y, Bebawy M: Isolation of Human CD138 (+) Microparticles from the Plasma of Patients with Multiple Myeloma. Neoplasia. 2016 Jan;18 (1) :25-32., URL: https://pubmed.ncbi.nlm.nih.gov/26806349/, *Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A: Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol. 1999 Jan;104 (1) :152-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/10027728, *Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20 (8) :1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/, *Use of this product has not been published., URL:

Other References

*Hayashida K, Johnston DR, Goldberger O, Park PW: Syndecan-1 expression in epithelial cells is induced by transforming growth factor beta through a PKA-dependent pathway. J Biol Chem. 2006 Aug 25;281 (34) :24365-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16807246?ordin, *Choi DS, Kim JH, Ryu HS, Kim HC, Han JH, Lee JS, Min CK: Syndecan-1, a key regulator of cell viability in endometrial cancer. Int J Cancer. 2007 Aug 15;121 (4) :741-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/17455248?ordin, *Manakil JF, Seymour GJ, Bartold PM: Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression. Oral Microbiol Immunol. 2007 Aug;22 (4) :272-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17600540?ordin, *Muto T, Miyoshi K, Munesue S, Nakada H, Okayama M, Matsuo T, Noma T: Differential expression of syndecan isoforms during mouse incisor amelogenesis. J Med Invest. 2007 Aug;54 (3-4) :331-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17878683?ordin, *Ojeh N, Hiilesvuo K, Wärri A, Salmivirta M, Henttinen T, Määttä A: Ectopic expression of syndecan-1 in basal epidermis affects keratinocyte proliferation and wound re-epithelialization. J Invest Dermatol. 2008 Jan;128 (1) :26-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/17625591?ordina, *Jilani I, Wei C, Bekele BN, Zhang ZJ, Keating M, Wierda W, Ferrajoli A, Estrov Z, Kantarjian H, O'Brien SM, Giles FJ, Albitar M: Soluble syndecan-1 (sCD138) as a prognostic factor independent of mutation status in patients with chronic lymphocytic leukemia. Int J Lab Hematol. 2008 Jan 7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18190591?ordina