Recombinant Human IL-7 Protein
Recombinant Human Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Asp26-His177 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-7; IL-7; IL7
UniProt
P13232
Expression Region
Asp26-His177
Tag
C-6His
Field of Research
Immunology & Inflammation
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
BLI: Loaded Mouse IL-7RA-Fc (Cat#orb2994406) on Protein A Biosensor, can bind Human IL-7-His (Cat#orb3002479) with an affinity constant of 29.0 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) . The ED50 for this effect is 50-300 pg/ml.
Form
Lyophilized
Molecular Weight
Predicted: 18.4 KDa. Observed: 19-30 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB,150mM NaCl,4%Mannitol, pH 7.4.
Available Sizes
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