Recombinant Human IL-2 Protein
Recombinant Human Interleukin-2 is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 is expressed with a 6His tag at the C-terminus.
Product Specifications
Product Name Alternative
Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2
UniProt
P60568
Expression Region
Ala21-Thr153
Tag
C-6His
Field of Research
Immunology & Inflammation
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity
BLI: Loaded Human IL-2RA-Fc (Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2-His (Cat#orb3002442) with an affinity constant of 3.94 nM as determined in BLI assay. Loaded Human IL-2RB-Fc (Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2-His (Cat#orb3002442) with an affinity constant of 0.17 uM as determined in BLI assay.. Cell Based Assay: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells The specific activity of Recombinant Human IL-2 is ≥1x107 IU/mg.
Form
Lyophilized
Molecular Weight
Predicted: 16.4 KDa. Observed: 14-18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 10mM Sodium Citrate, 4% Mannitol, pH 4.0
Available Sizes
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