Recombinant Human IL-7 Protein

Recombinant Human Interleukin-7 is produced by our E.coli expression system and the target gene encoding Asp26-His177 is expressed.

Product Specifications

Product Name Alternative

Interleukin-7; IL-7; IL7

UniProt

P13232

Expression Region

Asp26-His177

Tag

Tag free

Field of Research

Immunology & Inflammation

Endotoxin

< 0.01 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)

Bioactivity

BLI: Loaded Human IL-7RA-His (Cat#orb2993835) on HIS1K Biosensor, can bind Human IL-7 (Cat#orb3002429) with an affinity constant of 15.04 nM as determined in BLI assay. Loaded Human IL-7RA-Fc-His (Cat#orb2994563) on HIS1K Biosensor, can bind Human IL-7 (Cat#orb3002429) with an affinity constant of 18.59 nM as determined in BLI assay. Loaded Mouse IL-7RA-Fc (Cat#orb2994406) on Protein A Biosensor, can bind Human IL-7 (Cat#orb3002429) with an affinity constant of 29.2 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) . The ED50 for this effect is 0.02-0.08 ng/ml.

Form

Lyophilized

Molecular Weight

Predicted: 17.5 KDa. Observed: 18 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.