Recombinant Human RSPO2 Protein
Recombinant Human R-spondin-2 is produced by our Mammalian expression system and the target gene encoding Gln22-Gly205 is expressed with a Flag tag at the C-terminus.
Product Specifications
Product Name Alternative
R-spondin-2; RSPO2; Roof plate-specific spondin-2; hRspo2; PRO34209
UniProt
Q6UXX9
Expression Region
Gln22-Gly205
Tag
C-Flag
Field of Research
Stem Cell & Developmental Biology
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) . SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
Cell Based Assay: Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a V3 (Cat# orb3002512) . (Regularly tested)
Form
Lyophilized
Molecular Weight
Predicted: 22.3 KDa. Observed: 22-28 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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