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Recombinant Mouse PD-L1 Protein

Recombinant Mouse Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr238 is expressed with a 6His tag at the C-terminus.

Product Specifications

Product Name Alternative

Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; programmed death ligand 1; B7 homolog 1; B7-H1; CD274

UniProt

Q9EP73

Expression Region

Phe19-Thr238

Conjugation

Unconjugated

Tag

C-6His

Origin

Mouse

Field of Research

Immunology & Inflammation

Endotoxin

< 1 EU/μg as determined by LAL test.

Purity

SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

ELISA: Immobilized Anti-Human PDL1 mAb-Fc (Cat#orb2993144) at 2μg/ml (100 μl/well) can bind Mouse PD-L1-His (Cat#orb2994459) .The ED50 of Mouse PD-L1-His (Cat#orb2994459) is 23.35 ng/ml.. BLI: Loaded Mouse PD-L1-His (Cat#orb2994459) on HIS1K Biosensor, can bind Anti-Human PDL1 mAb-Fc (Cat#orb2993144) with an affinity constant of -3 nM as determined in BLI assay. (Regularly tested)

Form

Lyophilized

Molecular Weight

Predicted: 25.6 KDa. Observed: 39-58 KDa, reducing conditions

Storage Conditions

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Notes

For research use only.

Preservative

0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

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