Recombinant Human HER2 Protein (Biotin)
Biotinylated Recombinant Human Receptor tyrosine-protein kinase ErbB-2 is produced by our Mammalian expression system and the target gene encoding Thr23-Thr652 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.
Product Specifications
Product Name Alternative
Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Tyrosine kinase-type cell surface receptor HER2; ERBB2; MLN19; NGL; TKR1
UniProt
P04626
Expression Region
Thr23-Thr652
Conjugation
Biotin
Tag
C-Fc-Avi
Origin
Human
Field of Research
Signal Transduction
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Anti-Human HER2 mAb (Cat#orb2993152) at 2μg/ml (100 μl/well) can bind Human HER2-Fc-Avi (Cat#orb2993812) . The ED50 of Human HER2-Fc-Avi (Cat#orb2993812) is 3.07 ng/ml.. BLI: Loaded Biotinylated Human HER2-Fc-Avi (Cat#orb2993812) on SA Biosensor, can bind Anti-Human HER2 mAb (Cat#orb2993152) with an affinity constant of -3nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 98.3 KDa. Observed: 120-140 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
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