Recombinant Human APRIL Protein
Recombinant Human A proliferation-inducing Ligand is produced by our Mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, Flag tag at the N-terminus.
Product Specifications
Product Name Alternative
Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13
UniProt
O75888
Expression Region
Lys112-Leu250
Conjugation
Unconjugated
Tag
N-Flag-His
Origin
Human
Endotoxin
< 1 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
ELISA: Immobilized Human APRIL -Flag-His (Cat#orb2994084) at 1μg/ml (100 μl/well) can bind Cynomolgus BCMA-Fc (Cat#orb2994273) .The ED50 of Cynomolgus BCMA-Fc (Cat#orb2994273) is 4.23 ng/ml.. BLI: Loaded Cynomolgus BCMA-Fc (Cat#orb2994273) on Protein A Biosensor, can bind Human APRIL-His (Cat#orb2994084) with an affinity constant of 0.005 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 50 KDa. Observed: 60 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 1M NaCl, pH 8.3.
Available Sizes
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