Recombinant Human Laminin421 E8 Protein
Recombinant Human Laminin421 E8 is produced by our Mammalian expression system and the target gene encoding Glu629-His1449&Leu1573–Gln1798&Asn1364–Pro1609 is expressed with a 6His, Flag and HA tag at the N terminus respectively.
Product Specifications
Product Name Alternative
Laminin421; LN421
UniProt
P55268, P11047, Q16363
Expression Region
Glu629-His1449&Leu1573–Gln1798&Asn1364–Pro1609
Tag
N-6His&N-Flag&N-HA
Endotoxin
<0.01EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC Verified) .
Bioactivity
ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 1 Heterodimer (C-Flag, C-6His) (Cat#orb2992511) at 1μg/ml (100 μl/well) can bind Recombinant Human Laminin421 E8 Protein (Cat#orb3002509) *.The ED50 of Recombinant Human Laminin421 E8 Protein (Cat#orb3002509) is 0.70ng/ml. *: Biotinylated by NHS-biotin prior to testing. (Regularly tested) .
Form
Lyophilized
Molecular Weight
Predicted: 92.7&25.8&28.5kDa. Observed: (27-35) & (36-50) & (110-135) kDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of PBS, pH 7.4.
Available Sizes
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